Research infrastructure

(Bio-)organic synthesis

Sample preparation

Chemical and bioanalytics

Bioactivity studies

Computational studies

Table of Contents

(Bio-)organic synthesis

Sample preparation

Chemical and bioanalytics

Bioactivity studies

Computational studies

(Bio-)organic synthesis

Synthesis of modified fatty acids
manual synthesis, modification, and labeling
Contact person: Prof. Dr. Wolfgang Imhof (Organic Chemistry group)

Solid-phase Peptide Synthesis
manual synthesis microwave-assisted manual synthesis automated synthesis
Contact person: Jun.-Prof. Dr. Marie-T. Hopp (AG Bioorganische Chemie)

MultiPep 1 (Parallel peptide synthesizer, CEM): Automated solid-phase peptide synthesis, automated synthesis of peptide arrays on resin in 96-well filter plates and of membrane-bound peptide arrays (SPOT synthesis).

ETHOS lab (powerful microwave digestion system, MLS-MWS): Dual magnetron arrangement, MW input continuous or pulsed from 0 to 1600 watts, sample chamber volume of 70 liters (currently used for the synthesis of complex peptides).

Sample preparation

Digestion/extraction, concentration and drying of analytes
Extraction of microplastic Extraction of natural products Rotary evaporation lyophilization Drying in drying and vacuum drying cabinets Incubator Thermo shaker various centrifuges
Contact persons: Prof. Dr. Wolfgang Imhof (AG Organische Chemie) und Jun.-Prof. Dr. Marie-T. Hopp (AG Bioorganische Chemie)

EDGE (Solvant extraction system, CEM): Automated solvant extraction.

Alpha 2-4 LSCplus (Lyophilizer, Christ): 4 kg condensator capacity, -85°C condensator temperature, chemical resistant vacuum pump.

RVC 2-18 Cdplus (Rotary vacuum concentrator, Christ): HCl-resistant model with cold trap, use with 1.5/2 ml or 10 ml tubes possible.

Incucell (Incubator, MMM Medcenter): Accurate temperature control (30-100°C), natural air circulation, 55 l chamber volume.

VD56 (Vacuum drying cabinet, Bender): Temperature range: 9-220°C, pressure range: up to <10 mbar, 55 l chamber volume.

various drying cabinets:

Purification of proteins and peptides
FPLC (SEC)
Contact person: Jun.-Prof. Dr. Marie-T. Hopp (AG Bioorganische Chemie)

ÄKTA purifier (FPLC, SEC, Amersham/Cytiva): Protein purification system with UV detector. Different SEC columns available.

Chemical and bioanalytics

analytics of small molecules
Gas chromatography coupled to mass spectrometry (GC-MS) Nuclear magnetic resonance spectroscopy (NMR)
Contact person: Prof. Dr. Wolfgang Imhof (AG Organische Chemie)

ChromTech Evolution Mass spectrometer coupled to an Agilent Technologies 6890N Network GC System with autosampler.

Spinsolve 80 Ultra (Benchtop NMR, Magritek): 1H-Frequence: 80 MHz, Cores: 1H, 13C, 19F, PFG unit (DOSY), no deuterated solvents necessary (signal suppression), sequences (selection): 1D (decoupling), JRES, TOCSY, ROESY, WET, PRESAT, T1, T2, T2 filter, HSQC, HMQC, HMBC, DEPT, APT, Reaction monitoring

analytics of (in particular) peptides and proteins
Reversed-phase high-performance liquid chromatography (RP-HPLC) Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) UV/Vis spectroscopy Fluorescence spektroscopy
Contact person: Jun.-Prof. Dr. Marie-T. Hopp (AG Bioorganische Chemie)

RP-HPLC (follows soon...)

Specord 200 (UV/Vis spectrophotometer, Analytik Jena): Two-beam spectrophotometer, monochromator, wavelength range: 190-1100 nm, variable spectral bandwidth, wavelength accuracy: 0.5 nm, scanning speed: up to 6000 nm/min.

POLARstar Galaxy (Fluorescence Plate Reader, BMG Labtech): Absorbance, fluorescence intensity incl. FRET, time-resolved fluorescence (TRF), luminescence, fluorescence polarization.

Bioactivity studies

Chromogenic, fluorogenic, and Diagnostic Assay systems
UV/Vis spectroscopy Fluorescence spectroscopy Ball coagulometry
Contact person: Jun.-Prof. Dr. Marie-T. Hopp (AG Bioorganische Chemie)

Infinite 200 Pro (UV/Vis plate reader, Tecan): Spektrophotometer for microtiter plates or cuvettes, monochromator, wavelength range: 230-1000 nm.

POLARstar Galaxy (Fluorescence Plate Reader, BMG Labtech): Absorbance, fluorescence intensity incl. FRET, time-resolved fluorescence (TRF), luminescence, fluorescence polarization.

KC4A (4-channel coagulometer, Amelung): Blood coagulation measuring device (ball method), simultaneous measurement of 4 samples possible.

Computational studies

High-performance computer systems for calculations and simulations
Server for DFT calculations High-performance PCs for molecular docking and molecular dynamics simulations

AMD dual operton high performance server from DELTA, 4 server bays (each equipped with 2x 6212 AMD (Interlagos) 8-core CPU, 32 GB RAM), Gaussian program for DFT calculations

Hyundai-ITMC Pentino, Intel Core i5-12400 processor, 64 GB RAM DDR4 3200 MHz, 1 TB NVMe SSD, RTX A2000 6 GB graphics card, YASARA program, in silico simulation (peptides/proteins) and interaction studies